summary for prac microbe 2

practical summary of microb 2

kiri : Bacitracin test (transparent colony-B complete haemolysis)

sensitive: Streptococcus pyogene

resistant: other than Group A

kanan : Optochin test 

(grennish discolouration-alpha partial heamolysis)

terima kasih model tolong pegang! :)

closer view-Optochin test

sensitive : Streptococcus Pneumoniae/Pneumococci

resistance : Streptococci Viridans 

kiri : mannitol salt agar (MSA)-selective media

kanan : Staphylococci Aureus

-ferment mannitol produce colonies 

surrounded by yellow halloes-

COLONY ON BLOOD AGAR : ada 3

1. alpha haemolytic streptococci

2. beta heamolytic streptococci

3. beta haemolytic staphyloccoci

alpha haemolytic streptocci 

(greenish discolouration)

perhatikan 2 perbezaan di bawah ini!!

beta haemolytic streptoccoci

(translucent pin point no pigmented colony surrounded by large haemolysis)

 beta haemolytic staphylococci

(white large pigmented colony 

surrounded by small zone of haemolysis)

2nd point to be noticed : 

staph - non fastidious (both nutrient & blood agar)

strep - fastidious (only in blood agar)

see below!!

-sori gambar tak berapa jelas-

then, please recognize 4 things yang guna nutrient agar as medium:-

1. pigmented colony (kat atas)

2. Novobiocin test

resistance : Staphylococcus Saprophyticus

sensitive : Staphylococcus Epidermidis

3. antibiotic sensitivity test

disc diffusion method (staphylococci)

4.Phage typing

best method for typing Staphylococci

last test : HEAT RESISTANCE TEST

(Enterococcus fecalis)

E. faecalis can withstand heat at 60C for 30 mins

last but not least

blood culture media : DIPHASIC MEDIUM

slope of nutrient agar+10ml sulphonated broth

sekian untuk summary microbe 2

sori lambat

selamat study

:)