summary for prac microbe 2

practical summary of microb 2

kiri : Bacitracin test (transparent colony-B complete haemolysis)

sensitive: Streptococcus pyogene

resistant: other than Group A

kanan : Optochin test 

(grennish discolouration-alpha partial heamolysis)

terima kasih model tolong pegang! :)

closer view-Optochin test

sensitive : Streptococcus Pneumoniae/Pneumococci

resistance : Streptococci Viridans 

kiri : mannitol salt agar (MSA)-selective media

kanan : Staphylococci Aureus

-ferment mannitol produce colonies 

surrounded by yellow halloes-

COLONY ON BLOOD AGAR : ada 3

1. alpha haemolytic streptococci

2. beta heamolytic streptococci

3. beta haemolytic staphyloccoci

alpha haemolytic streptocci 

(greenish discolouration)

perhatikan 2 perbezaan di bawah ini!!

beta haemolytic streptoccoci

(translucent pin point no pigmented colony surrounded by large haemolysis)

 beta haemolytic staphylococci

(white large pigmented colony 

surrounded by small zone of haemolysis)

2nd point to be noticed : 

staph - non fastidious (both nutrient & blood agar)

strep - fastidious (only in blood agar)

see below!!

-sori gambar tak berapa jelas-

then, please recognize 4 things yang guna nutrient agar as medium:-

1. pigmented colony (kat atas)

2. Novobiocin test

resistance : Staphylococcus Saprophyticus

sensitive : Staphylococcus Epidermidis

3. antibiotic sensitivity test

disc diffusion method (staphylococci)

4.Phage typing

best method for typing Staphylococci

last test : HEAT RESISTANCE TEST

(Enterococcus fecalis)

E. faecalis can withstand heat at 60C for 30 mins

last but not least

blood culture media : DIPHASIC MEDIUM

slope of nutrient agar+10ml sulphonated broth

sekian untuk summary microbe 2

sori lambat

selamat study

:)

histology of BVs ( slide )

slide :
1) AORTA ( H&E, orcein & VVG )
2) MED SIZED A&V ( H&E, orcein, VVG )



AORTA ( H&E )



AORTA (ORCEIN)



AORTA ( VVG )



MEDIUM-SIZED A&V ( H&E )



MEDIUM-SIZED A&V ( VVG )



MEDIUM-SIZED A&V ( ORCEIN )

p/s : orcein stain utk med sized a&v kte amik kt tenet. tyme amik gmba kt aifa ri2, xprasan termiss gmba tu. sowie. kalo nk lebih accurate, bley rujuk slide histo alexmed :)

histo gambar BV

as salam.

saya menjalankan tugas.

slide histo. tak ade slide microscope.

gambar kat file ja :)

medium sized vein and artery.

thick wall : artery [ kanan sekali ]

thin wall : vein [kiri sekali]

nak bezakan artery and vein :

artery ada IEL.

internal elastic lamina.

yang ade fold sangat obvious.

1 : tunica intima

2 : tunica media

3 : tunica adventitia.

what are cardiac tamponade & aneurysm (pericardial disease)

Aneurysms

source : http://nursingcrib.com/nursing-notes-reviewer/aortic-aneurysm/

can commonly occur in arteries at the base of the brain (the circle of Willis) and an aortic aneurysm occurs in the main artery carrying blood from the left ventricle of the heart. When the size of an aneurysm increases, there is a significant risk of rupture, resulting in severe hemorrhage, other complications or death. Aneurysms can be hereditary or caused by disease, both of which cause the wall of the blood vessel to weaken






cardiac tamponade

Cardiac tamponade, also known as pericardial tamponade, is an emergency condition in which fluid accumulates in the pericardium (the sac in which the heart is enclosed). If the fluid significantly elevates the pressure on the heart it will prevent the heart's ventricles from filling properly. This in turn leads to a low stroke volume. The end result is ineffective pumping of blood,shock, and often death.

Cardiac tamponade occurs when the pericardial space fills up with fluid faster than the pericardial sac can stretch. If the amount of fluid increases slowly (such as in hypothyroidism) the pericardial sac can expand to contain a liter or more of fluid prior to tamponade occurring. If the fluid occurs rapidly (as may occur after trauma or myocardial rupture) as little as 100 ml can cause tamponade.

aortic arch & aorticopulmonary window

aorticopulmonary window

cardiac cycle :)

salam.
link kt bwh ni ade sket NOTES utk CARDIAC CYCLE yg kte bru blaja arini.
there are some pics & video for better understanding, insyaAllah. jenguk2 la ye :))
All the phases are included, PHASE1 - PHASE7. same mcm dr ajar td ",)

http://www.cvphysiology.com/Heart%20Disease/HD002.htm

Ya Allah, permudahkanlah kami utk memahami ilmuMU... :)

ECG

here some useful videos.take a look peeps

lagi satu

FADZLIN SAKINA with love

prak microb I

as salam

mak cik-mak cik dayang sekalian.

ni praktikal microb punya gambar. kesian kat esah asyek dia je upload.

tolonglah sama2 skali ya.

insyallah PAHALA.

peptone water.

nutrient agar slope.

nutrient broth.

sugar fermentation -ve RED

sugar fermentation + ve YELLOW with acid production.

 sugar fermentation + ve YELLOW with acid production.

ada inverted durham's tube so : ada gas sekali.

soft agar.

deep agar.

media pulak.

mannitol salt agar.

chocolate agar.

nutrient agar.

bacitracin test.

baca sendiri kat atas. hahahaha:))